The genome sequence of the Dracula fish, Danionella dracula (Britz, Conway & Rüber, 2009)

We present a genome assembly from an individual Danionella dracula (the Dracula fish; Chordata; Actinopterygii; Cypriniformes; Danionidae; Danioninae). The genome sequence is 665.21 megabases in span. This is a scaffold-level assembly, with a scaffold N50 of 10.29 Mb.

Danionella dracula has recently emerged as a model organism for neurophysiological research (Tatarsky et al., 2022), along with D. cerebrum (Schulze et al., 2018), previously misidentified as D. translucida (Britz et al., 2021).A hybrid genome assembly for D. cerebrum has previously been published (Kadobianskyi et al., 2019), and we complement this with publication of the D. dracula genome here.
Danionella dracula shows a number of highly unusual features that have been the prerequisite for its establishment as a neurophysiological model organism.The skeleton of Danionella dracula is characterised by loss and reduction of 61 bones, bone parts or cartilages (Britz & Conway, 2016), including the frontals and parietals, which form the skull roof in other bony fishes.Danionella dracula is one of the most developmentally truncated fishes, meaning that its anatomy, with few exceptions, resembles that of a larval fish.This organism-wide progenetic condition has facilitated the evolution of several morphological novelties (Britz & Conway, 2016), most of them sexually dimorphic.The most dramatic sexual dimorphic novelties involve its jaws, with large males developing tooth-like fangs, and its Weberian apparatus, in which males have hypertrophied elements and a bulbous drumming muscle and cartilage (Britz et al., 2009;Britz & Conway, 2016).Both these character complexes are also exceptions to the organism-wide progenesis, and at least the well-developed Weberian apparatus is the result of striking heterochronic changes during the ontogeny of D. dracula (Conway et al., 2021).While the development of most of the skeleton of D. dracula is delayed when compared to the zebrafish and other cypriniforms, that of the components of the Weberian apparatus is greatly accelerated resulting in a larval-looking fish with a well-developed Weberian apparatus used for hearing and sound production (Conway et al., 2021).This highly unusual combination of an astonishingly transparent body, a reduced skeleton with no skull roof allowing immediate access to one of the smallest vertebrate brains, and a highly developed sound reception and production system combined with a complex behavioural repertoire have made this fish an attractive research subject.
Danionella dracula is a member of the Danioninae and a close relative of the zebrafish Danio rerio.The Danioninae are an emerging model clade in the field of evo-devo, favoured for analyses of anatomy (especially bone morphology and growth), pigmentation, gene family expansion and phylogeography (Braasch et al., 2015;Parichy, 2015).Within the Danioninae Sequencing Project we are providing high quality genome assemblies for representatives of this clade to facilitate its use.

Genome sequence report
The specimen used for the study was obtained through the aquarium trade.The species naturally occurs in a stream near Sha Du Zup between Mogaung and Tanai, Myitkina district, Kachin State, northern Myanmar.A total of 41-fold coverage in Pacific Biosciences single-molecule continuous long reads (CLR) and 104-fold coverage in 10X Genomics read clouds were generated.The final assembly has a total length of 665.21 Mb in 996 sequence scaffolds with a scaffold N50 of 10.29 Mb (Table 1, Figure 2, Figure 3 and Figure 4).
Metadata for spectra estimates and sequencing runs can be found at https://links.tol.sanger.ac.uk/species/623740.

Sample acquisition and nucleic acid extraction
The Danionella dracula specimen used for the genome assembly (BioSample ID SAMEA104026433, individual fDanDra1), was obtained from the laboratory of Ralf Britz.Individuals of D. dracula were maintained in an aquarium with the dimensions 80×40×40 cm and fed with brine shrimp nauplii.Specimens were euthanised with an overdose of MS222.Tissue was flash-frozen on dry ice.Two different DNA extraction methods were applied for the fDanDra1 sample.DNA was then extracted from head tissue using a modified version of the MagAttract protocol to increase the yield.HMW DNA was sheared into an average fragment size of 12-20 kb in a Megaruptor 3 system with speed setting 30.Sheared DNA was purified by solid-phase reversible immobilisation using AMPure PB beads with a 1.8X ratio of beads to sample to remove the shorter fragments and concentrate the DNA sample.
The concentration of the sheared and purified DNA was assessed using a Nanodrop spectrophotometer and Qubit Fluorometer and Qubit dsDNA High Sensitivity Assay kit.
Fragment size distribution was evaluated by running the sample on the FemtoPulse system.

Sequencing
Pacific Biosciences circular consensus and 10X Genomics read cloud DNA sequencing libraries were constructed according to the manufacturers' instructions.DNA sequencing was performed by the Scientific Operations core at the WSI on Pacific Biosciences SEQUEL (CLR) and HiSeqX (10X) instruments.

Genome assembly, curation and evaluation
The assembly fDanDra1.1 is based on PacBio Sequel data, and Illumina HiSeqX data generated from a 10X Genomics Chromium library.An initial PacBio assembly was made using Falcon-unzip (Chin et al., 2016).The primary contigs were first scaffolded using a wtdbg (Ruan & Li, 2020) assembly as a synteny guide, then scaffolded further using the 10X data Finally, the assembly was manually assessed using gEVAL (Chow et al., 2016).
Table 2 lists the relevant software tool versions and sources.

Wellcome Sanger Institute -Legal and Governance
The materials that have contributed to this genome note have been supplied by a Tree of Life collaborator.The Wellcome Sanger Institute employs a process whereby due diligence is carried out proportionate to the nature of the materials themselves, and the circumstances under which they have been/are to be collected and provided for use.The purpose of this is to address and mitigate any potential legal and/or ethical implications of receipt and use of the materials as part of the research project, and to ensure that in doing so we align with best practice wherever possible.The overarching areas of consideration are: • Ethical review of provenance and sourcing of the material   Table 2. Software tools: versions and sources.

Software tool Version
released openly for reuse.The Danionella dracula genome sequencing initiative is part of the Vertebrate Genomes Project.All raw sequence data and the assembly have been deposited in INSDC databases.Raw data and assembly accession identifiers are reported in Table 1.
The reference genome is well described and clearly presented.There is logical flow and appropriate level of detail.The approaches applied are well motivated and sufficiently comprehensive for the purpose of releasing this useful resource.I have no further requests for clarification and I look forward to seeing this taken up by the community

Chenhong Li
Shanghai Ocean University, Shanghai, China Dracula fish is a interesting fish the only one with fangs in carp species.The data generated are very useful.All analyses and information extraction were done appropriately.The species itself is unique.The genome resource data collected here will be valuable to further investigate this creature and to serve the purpose of using it as a model organism.

Is the rationale for creating the dataset(s) clearly described? Yes
Are the protocols appropriate and is the work technically sound?Yes

Are sufficient details of methods and materials provided to allow replication by others? Yes
Are the datasets clearly presented in a useable and accessible format?Yes Competing Interests: No competing interests were disclosed.
Reviewer Expertise: Phylogenetics, comparative genomics, fish I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard.
In the manuscript titled "The genome sequence of the Dracula fish, Danionella dracula (Britz, Conway & Rüber, 2009)" by Rüber et al. the authors sequenced and assembled a scaffold-level genome of Dracula fish.They describe the importance of this resource as a model of neurophysiological research, and improve the genome quality compared to the previously published hybrid assembly.The manuscript is well written and the authors provide most relevant details.The workflow for the assembly assessment is cited including the list of tools/software for the assembly.However, the code/scripts used to generate the actual assembly is missing in the references and availability statement.It would be of great help to the research community for reproducibility if these details (data-specific parameters) can be provided through relevant Zenodo/Git repositories.If default parameters were used for all the tools, please mention it specifically in the methods section.
Is the rationale for creating the dataset(s) clearly described?Yes Are the protocols appropriate and is the work technically sound?Yes

Are sufficient details of methods and materials provided to allow replication by others? No
Are the datasets clearly presented in a useable and accessible format?Yes Competing Interests: No competing interests were disclosed.
Reviewer Expertise: Genomics, Bioinformatics I confirm that I have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard.

Figure 2 .
Figure 2. Genome assembly of Danionella dracula, fDanDra1.1:metrics.The BlobToolKit Snailplot shows N50 metrics and BUSCO gene completeness.The main plot is divided into 1,000 size-ordered bins around the circumference with each bin representing 0.1% of the 665,208,374 bp assembly.The distribution of scaffold lengths is shown in dark grey with the plot radius scaled to the longest scaffold present in the assembly (39,982,851 bp, shown in red).Orange and pale-orange arcs show the N50 and N90 scaffold lengths (10,287,669 and 757,246 bp), respectively.The pale grey spiral shows the cumulative scaffold count on a log scale with white scale lines showing successive orders of magnitude.The blue and pale-blue area around the outside of the plot shows the distribution of GC, AT and N percentages in the same bins as the inner plot.A summary of complete, fragmented, duplicated and missing BUSCO genes in the actinopterygii_odb10 set is shown in the top right.An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/fDanDra1.1/dataset/UELW01/snail.

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Legality of collection, transfer and use (national and international).Each transfer of samples is undertaken according to a Research Collaboration Agreement or Material Transfer Agreement entered into by the Tree of Life collaborator, Genome Research Limited (operating as the Wellcome Sanger Institute) and in some circumstances other Tree of Life collaborators.

Figure 3 .
Figure 3. Genome assembly of Danionella dracula, fDanDra1.1:BlobToolKit GC-coverage plot.Scaffolds are coloured by phylum.Circles are sized in proportion to scaffold length.Histograms show the distribution of scaffold length sum along each axis.An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/fDanDra1.1/dataset/UELW01/blob.

Figure 4 .
Figure 4. Genome assembly of Danionella dracula, fDanDra1.1:BlobToolKit cumulative sequence plot.The grey line shows cumulative length for all scaffolds.Coloured lines show cumulative lengths of scaffolds assigned to each phylum using the buscogenes taxrule.An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/fDanDra1.1/dataset/UELW01/cumulative.

Is the rationale for creating the dataset(s) clearly described? Yes Are the protocols appropriate and is the work technically sound? Yes Are sufficient details of methods and materials provided to allow replication by others? Yes Are the datasets clearly presented in a useable and accessible format? Yes Competing Interests:
No competing interests were disclosed.

have read this submission and believe that I have an appropriate level of expertise to confirm that it is of an acceptable scientific standard.
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